分子量:44.4 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Osteoprotegerin, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its binding ability in a functional ELISA. Immobilized rh TNFRSF11B at 10 μg/mL (100 μl/well) can bind human TNFSF11 Fc Chimera with a linear ranger of 4-256 ng/mL.

产品特性:Human Osteoprotegerin, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 44.4 kDa. The predicted N-terminus is Glu 22. The reducing (R) protein migrates as 55 kDa in SDS-PAGE  due to glycosylation.

产品背景:Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) is also known as Osteoclastogenesis inhibitory factor (OCIF), Osteoprotegerin (OPG). TNFRSF11B is a secreted homodimer protein, which can interact with TNFSF10 and TNFSF11. TNFRSF11B acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.