ID1 Validated Chimera RNAi
产品名称： ID1 Validated Chimera RNAi
英文名称： ID1 Validated Chimera RNAi
产品编号： H00003397-R01V
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Homo sapiens inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), transcript variant 1, mRNA.
- Reactivity:
- Human
- Storage Instruction:
- Store at -20°C, do not exceed 4 - 5 freeze-thaw cycles to ensure product integrity.
- Supplied Product:
- DEPC water
- Target Refseq:
- NM_002165
- Note:
- Position of the Chimera RNAi.
-
- Publication Reference
- 1.
- dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference.
Naito Y, Yamada T, Matsumiya T, Ui-Tei K, Saigo K, Morishita S.Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W589-91.
- 2.
- Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect.
Ui-Tei K, Naito Y, Zenno S, Nishi K, Yamato K, Takahashi F, Juni A, Saigo K.Nucleic Acids Res. 2008 Apr;36(7):2136-51. Epub 2008 Feb 11.
- 3.
- Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference.
Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K.Nucleic Acids Res. 2004 Feb 9;32(3):936-48. Print 2004.
- 4.
- siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference.
Naito Y, Yamada T, Ui-Tei K, Morishita S, Saigo K.Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W124-9.
- Applications
- RNAi Knockdown (Antibody validated)
- Western blot analysis of ID1 over-expressed 293 cell line, cotransfected with ID1 Validated Chimera RNAi ( Cat # H00003397-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID1 monoclonal antibody (M02), clone 1F7 (Cat # H00003397-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 3397
- Gene Name:
- ID1
- Gene Alias:
- ID,bHLHb24
- Gene Description:
- inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
- Omim ID:
- 600349
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- DNA-binding protein inhibitor ID-1,OTTHUMP00000030534,OTTHUMP00000030535,dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein),inhibitor of DNA binding 1,inhibitor of differentiation 1
- Gene Pathway