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上海信裕生物科技有限公司
抗体

抗体

商家询价

产品名称: 抗体

英文名称: EIF4G1Antibody

产品编号: XY15704-1

产品价格: null

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: WB,ELISA,IHC-P,IHC-F,IF

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 EIF4G1抗体

Applications
In-house applications ELISA, WB, IHC, IF, IP     Application not tested? Please see our Guarantee.
Published applications WB Publications 3 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 15704-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP(EIF4G1 antibody) at dilution of 1:1000
  • 15704-1-AP;human breast cancerImmunohistochemical of paraffin-embedded human breast cancer using 15704-1-AP(EIF4G1 antibody) at dilution of 1:100 (under 40x lens)
  • 15704-1-AP;HelaImmunofluorescent analysis of Hela cells, using EIF4G1 antibody 15704-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
  • 15704-1-AP;HeLa cellsIP Result of anti-EIF4G1 (IP:15704-1-AP, 5ug; Detection:15704-1-AP 1:500) with HeLa cells lysate 2000ug.
Positive WB detected in HeLa cells
Positive IP detected in HeLa cells
Positive IHC detected in Human breast cancer tissue
Positive IF detected in Hela cells, Ethacrynic acid treated HepG2 cells, HepG2 cells
Recommended dilution WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
 EIF4G1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen EIF4G1 fusion protein ag8342 Full name Eukaryotic translation initiation factor 4 gamma, 1
Calculated molecular
weight
1600aa,176 kDa Observed molecular 
weight
250 kDa
GenBank accession 
number
BC007788 Gene ID (NCBI) 1981
Gene symbol EIF4G1 Synonyms DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220
Background EIF4G1抗体

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. EIF4G1抗体

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