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上海信裕生物科技有限公司
KAP1抗体

KAP1抗体

商家询价

产品名称： KAP1抗体

英文名称： KAP1Antibody

产品编号： XY15202-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 KAP1抗体

Applications
In-house applications ELISA, WB, IHC, IF, FC, IP     Application not tested? Please see our Guarantee.
Published applications IHC, WB Publications 2 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human, mouse
Images
Prev12NextView more validation data
  • 15202-1-AP;HeLa cellHeLa cells were subjected to SDS PAGE followed by western blot with 15202-1-AP(KAP1 antibody) at dilution of 1:3000
  • 15202-1-AP;Colon cancerImmunohistochemical of paraffin-embedded human colon cancer using 15202-1-AP(KAP1 antibody) at dilution of 1:100 (under 10x lens)
  • 15202-1-AP;Colon cancerImmunohistochemical of paraffin-embedded human colon cancer using 15202-1-AP(KAP1 antibody) at dilution of 1:100 (under 40x lens)
  • 15202-1-AP;HelaImmunofluorescent analysis of Hela cells, using TRIM28 antibody 15202-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
  • 15202-1-AP;HeLa cellsIP Result of anti-KAP1 (IP:15202-1-AP, 4ug; Detection:15202-1-AP 1:1000) with HeLa cells lysate 1200ug.
  • 15202-1-AP;HeLa cells1X10^6 HeLa cells were stained with 0.2ug KAP1 antibody (15202-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in HeLa cells, mouse testis tissue, rat spleen tissue
Positive IP detected in HeLa cells
Positive IHC detected in Human colon cancer tissue
Positive IF detected in Hela cells
Positive FC detected in HeLa cells
Recommended dilution WB : 1:1000-1:10000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100
 KAP1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen KAP1 fusion protein ag7350 Full name Tripartite motif-containing 28
Calculated molecular
weight		 89 kDa Observed molecular 
weight		 100kd
GenBank accession 
number		 BC004978 Gene ID (NCBI) 10155
Gene symbol TRIM28 Synonyms FLJ29029, KAP 1, KAP1, KRAB associated protein 1, KRAB interacting protein 1, KRIP 1, Nuclear corepressor KAP 1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1B, TRIM28, tripartite motif containing 28
XY202Hu21 氧化应激诱导生长抑制因子1(OSGIN1)单克隆抗体 Monoclonal Antibody to Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) Homo sapiens (Hukl)
XY236Hu21 酰基甘油激酶(AGK)单克隆抗体 Monoclonal Antibody to Acylglycerol Kinase (AGK) Homo sapiens (Hukl)
XY239Hu21 外生骨疣蛋白1(EXT1)单克隆抗体 Monoclonal Antibody to Exostoses 1 (EXT1) Homo sapiens (Hukl)
XY246Hu21 蛋白聚糖4(PRG4)单克隆抗体 Monoclonal Antibody to Proteoglycan 4 (PRG4) Homo sapiens (Hukl)
XY260Hu21 蛋白酶体亚基α5(PSMα5)单克隆抗体 Monoclonal Antibody to Proteasome Subunit Alpha Type 5 (PSMa5) Homo sapiens (Hukl)
XY262Hu21 蛋白酶体亚基α7(PSMα7)单克隆抗体 Monoclonal Antibody to Proteasome Subunit Alpha Type 7 (PSMa7) Homo sapiens (Hukl)
XY273Hu21 蛋白酶体亚基β6(PSMβ6)单克隆抗体 Monoclonal Antibody to Proteasome Subunit Beta Type 6 (PSMb6) Homo sapiens (Hukl)
XY283Hu21 蛋白酶激活亚基3(PSME3)单克隆抗体 Monoclonal Antibody to Proteasome Activator Subunit 3 (PSME3) Homo sapiens (Hukl)
XY291Hu21 鸟苷酸环化酶激活因子2A(GUCA2A)单克隆抗体 Monoclonal Antibody to Guanylate Cyclase Activator 2A (GUCA2A) Homo sapiens (Hukl)
XY292Hu21 鸟苷酸环化酶激活因子2B(GUCA2B)单克隆抗体 Monoclonal Antibody to Guanylate Cyclase Activator 2B (GUCA2B) Homo sapiens (Hukl)
XY300Hu21 小脑肽1(CBLN1)单克隆抗体 Monoclonal Antibody to Cerebellin 1 (CBLN1) Homo sapiens (Hukl)
XY301Hu21 黑素瘤缺乏因子1(AIM1)单克隆抗体 Monoclonal Antibody to Absent In Melanoma 1 (AIM1) Homo sapiens (Hukl)
XY302Hu21 黑素瘤缺乏因子2(AIM2)单克隆抗体 Monoclonal Antibody to Absent In Melanoma 2 (AIM2) Homo sapiens (Hukl)
XY304Hu21 磷酸甘油酸酯激酶1(PGK1)单克隆抗体 Monoclonal Antibody to Phosphoglycerate Kinase 1 (PGK1) Homo sapiens (Hukl)
XY307Hu21 多胺调节因子1结合蛋白1(PMFBP1)单克隆抗体 Monoclonal Antibody to Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1) Homo sapiens (Hukl)
XY313Hu21 半胱氨酸丰富血管生成诱导因子61(CYR61)单克隆抗体 Monoclonal Antibody to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) Homo sapiens (Hukl)
XY316Hu21 Corin蛋白(CRN)单克隆抗体 Monoclonal Antibody to Corin (CRN) Homo sapiens (Hukl)
XY316Ra21 Corin蛋白(CRN)单克隆抗体 Monoclonal Antibody to Corin (CRN) Rattus norvegicus (Rat)
XY321Hu21 丝氨酸蛋白酶8(PRSS8)单克隆抗体 Monoclonal Antibody to Protease, Serine 8 (PRSS8) Homo sapiens (Hukl)
Background KAP1抗体

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. KAP1抗体